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J Transl Genet Genom 2021;5:250-264.10.20517/jtgg.2021.23© The Author(s) 2021.
Open AccessOriginal Article

Wide-field optical coherence tomography in ABCA4-associated inherited retinal dystrophies

1Rare Retinal Disease Center, AugenZentrum Siegburg, MVZ Augenärztliches Diagnostik- und Therapiezentrum Siegburg GmbH, Siegburg 53721, Germany.

2Institute of Human Genetics, University of Regensburg, Regensburg 93053, Germany.

3RetinaScience, Bonn 53192, Germany.

4Institute of Clinical Human Genetics, University Hospital Regensburg, Regensburg 93053, Germany.

5Eye Care Center at St. Franziskus Hospital Münster, Münster 48145, Germany.

6Achim-Wessing-Institute for Ophthalmologic Diagnostics, University Hospital Essen, Essen 45147, Germany.

Correspondence to: Mohammad Saleh, Rare Retinal Disease Center, AugenZentrum Siegburg, MVZ Augenärztliches Diagnostik- und Therapiezentrum Siegburg GmbH, Europaplatz 3, Siegburg 53721, Germany. E-mail:

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    Academic Editor: Sanjay Gupta | Copy Editor: Yue-Yue Zhang | Production Editor: Yue-Yue Zhang

    © The Author(s) 2021. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License (, which permits unrestricted use, sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.


    Aim: With a need to expand the monitoring options in therapeutic clinical trials, we evaluated the additional information provided by wide-field optical coherence tomography (W-OCT) compared to conventional macular volume scan OCT (M-OCT) in ABCA4 gene-associated inherited retinal dystrophies (ABCA4-IRD).

    Methods: A consecutive series of 52 ABCA4-IRD patients (mean age at last examination: 35.9 years, range 8.8-68.7 years) was examined between 2015 and 2021. Ophthalmologic examination included clinical examination, M-OCT [20 × 20 degree field (6.2 mm × 6.2 mm)], W-OCT [55 × 25 degree field (16.1 mm × 7.3 mm)], multicolor reflectance photography, fundus (FAF), and near-infrared autofluorescence (NIA) in macular and wide-field mode. Molecular genetic testing to confirm the clinical phenotype was performed in all patients.

    Results: In 37/52 (71.2%) of patients W-OCT revealed alterations of the outer retinal layers beyond the area covered by M-OCT at their last examination. In 15 patients, lesions were located within the area covered by M-OCT. Lesions beyond M-OCT consisted of subretinal material (31/37), as well as patches (18/37) or large continuous areas (3/37) of photoreceptor and retinal pigment epithelial dystrophy. In one patient, W-OCT identified peripheral lesions that were not detectable in wide-field FAF and NIA. In 48/52 patients, two causative mutations in the ABCA4 gene were identified, while the remaining four patients carried one pathogenic ABCA4 variant.

    Conclusion: W-OCT as well as wide-field FAF and NIA document lesions in the retinal mid- and far periphery in the majority of ABCA4-IRD patients and provide means for detailed analysis of progression and future treatment planning and monitoring.


    ABCA4-associated inherited retinal dystrophies (ABCA4-IRD) present predominantly as autosomal recessive macular dystrophy (Stargardt disease) or autosomal recessive cone-rod dystrophy, but they can also present as autosomal recessive retinitis pigmentosa, autosomal recessive cone dystrophy, and age-related macular degeneration type 2[1]. Thus far, 1530 genetic variants have been described in the ABCA4 gene and classified as variants of disparate severity on the clinical phenotype expression[2-4]. Although clinical manifestations of ABCA4-IRD can be variable, lesions are usually similar in both eyes of one patient. Generally, fleck-like lesions and atrophic alterations initially manifest at the posterior pole, and during progression areas outside of the macula present alterations visible on ophthalmoscopy.

    Detailed retinal imaging, identifying more lesions than are clinically visible, has demonstrated characteristic alterations of ABCA4-IRD on fundus autofluorescence (FAF), near-infrared autofluorescence (NIA), and optical coherence tomography (OCT)[5-8]. Typical findings are fleck-like lesions with increased or reduced FAF or NIA intensity, partially corresponding to subretinal material (SRM) on OCT as well as focal or widespread areas with markedly reduced FAF and NIA intensity corresponding to photoreceptor and retinal pigment epithelial (RPE) loss on OCT. Whereas wide-field imaging for FAF and NIA has been used for more than 16 years, wide-field OCT (W-OCT) extending peripherally of the macular area has only become available recently. Until now, the relevance of W-OCT imaging has predominantly been evaluated with respect to repeatability of measurements[9]; normal retinal structures, especially the nerve fiber layer and choroidal layers[10-17]; and glaucoma-associated pathologies[18-22]. Studies regarding retinal disorders focused on diabetic retinopathy[23-27], central serous chorioretinopathy[28], or alterations associated with myopia[29,30], whereas retinal dystrophies have rarely been examined by W-OCT[31-34] and, if so, only in small patient series. When comparing these W-OCT studies, it has to be kept in mind that the terms “wide-field” and “ultra-wide-field” have been used for similar ranges of measured areas, and sometimes the measured area has not been reported and can only be surmised from the presented images.

    In the present study, we evaluated the information provided by W-OCT [55 × 25 degree field (16.1 mm × 7.3 mm)] compared to conventional macular OCT [M-OCT; 20 × 20 degree field (6.2 mm × 6.2 mm)] in a large series of genetically confirmed ABCA4-IRD patients.


    Included in this study was a consecutive series of unrelated patients with ABCA4-IRD who underwent W-OCT imaging during their initial or follow-up visit in a specialized IRD center between September 2015 and January 2021. ABCA4-IRD was defined as the presence of two likely pathogenic or pathogenic ABCA4 gene mutations or a single likely pathogenic or pathogenic ABCA4 gene mutation with clinical findings consistent with ABCA4-IRD [Table 1]. ABCA4 variants were classified based on the standards and guidelines recommended by the American College of Medical Genetics and Genomics and the Association for Molecular Pathology[35]. Molecular genetic testing was performed in 9 patients by ABCR genotyping microarray (ABCR400 chip)[36], 2 patients by a custom designed resequencing array (RetChip)[4], and 41 patients by Next Generation Sequencing using targeted gene panels including ABCA4 and a variable number of other genes that have been associated with macular dystrophy[4]. Additional causal sequence changes in other genes than ABCA4 have not been identified in this group of patients.

    Table 1

    ABCA4 gene sequence variants identified in 52 patients with IRD

    Patient_IDAllel 1 (DNA)Allel 1 (Protein)Allel 2 (DNA)Allel 2 (Protein)Group
    3c.768G>Tp.[(Val256=),(Leu257Valfs*17)] c.5882G>Ap.(Gly1961Glu)2
    5c.5413A>Gp.(Asn1805Asp)c.4880_4903dupp.(Leu1627_Ala1634dup) 3
    17c.2588G>Cp.(Gly863Ala, G863del)c.3259G>Ap.(Glu1087Asp)1
    29c.2588G>C(;)5603A>Tp.[Gly863Ala,Gly863del](;)(Asn1868Ile)c.2588G>Cp.(Gly863Ala, Gly863del)1
    37c.454C>T(;)6148G>Cp.(Arg152*)c.656G>C(;)2588G>C(;)5603A>Tp.[(Arg219Thr)(;)(Gly863Ala, G863del)(;)(Asn1868Ile)3

    All patients underwent clinical ophthalmological examination as well as detailed retinal imaging following informed consent after detailed explanation about the background of the study. The study was performed in adherence to the tenets of the Declaration of Helsinki. All applicable institutional and governmental regulations concerning the ethical use of human volunteers were followed during this research.

    Retinal imaging

    Retinal imaging including multicolor spectral reflectance imaging, FAF, NIA, and OCT were performed as described previously[5]. All images were obtained after medical dilatation of the pupil (phenylephrine 2.5% and tropicamide 1% achieving a minimal diameter of 5 mm) by trained retinal imaging specialists. FAF and NIA were obtained with a confocal scanning laser ophthalmoscope (Heidelberg Retina Angiograph 2, Heidelberg Engineering, Heidelberg, Germany) using 30° (M-FAF, M-NIA) and 55° lenses (W-FAF, W-NIA). Multicolor spectral reflectance images and OCT were obtained with a spectral domain OCT (Spectralis OCT, Heidelberg Engineering, Heidelberg, Germany). A standard volume scan macular OCT (M-OCT) was recorded using 49 B-scans with a distance between B-scans of 129 µm in a 20 × 20 degree field (6.2 mm × 6.2 mm) using ART mode with 16 images averaged. A wide-field (W-OCT) was recorded using 31 B-scans with a distance between B-scans of 245 µm in a 55 × 25 degree field (16.1 mm × 7.3 mm) using ART mode with 16 images averaged. All images were evaluated by two observers (Saleh M and Kellner U), and W-OCT images were compared to M-OCT as well as M-/W-FAF and M-/W-NIA findings. Lesions detected on retinal imaging were similar on both eyes in nearly all patients; therefore, the results are presented combined for both eyes. In two patients (#38 and #52), the results of the more severely affected eye were selected. In addition, unilateral focal choroidal excavation was identified in three patients (#12, #44, and #48).

    Statistical analysis

    Correlation of the ABCA4 genotype with lesions detected by W-OCT was determined by applying a Fisher’s exact test for count data implemented in the statistical programming software R[37]. Differences in age distributions were evaluated by a two-sided t test for independent samples by the t test function implemented in R. Statistical significance was defined by a P-value below 0.05.


    Fifty-two unrelated ABCA4-IRD patients were included in this study (32 females, 20 males; Table 2). The age at first examination ranged 7-66 years, and it ranged 8-68 years at last examination. W-OCTs could be recorded in 50/52 patients using 31 B-scans. Due to problems with fixation, in one patient the scan pattern was reduced to 11 B-scans and in one patient only single B-scans were possible. Clinical findings as well as retinal imaging results identified similar characteristics in both eyes of all patients except when specifically mentioned.

    Table 2

    Retinal imaging findings at last examination

    Patient_IDAge last exam (year)Follow-up (year)M_OCTW_OCTM_FAFW_FAFPSM_NIAW_NIAPS
    546.00AA, post staphylomaAA, FIPSARPS
    1041.50ANormal, dome-shaped FINormalNPIRRPS
    2429.70ANormalRI, FINormalNPINDNDND
    3143.80AScalloped WAAA, FINoPSAMRND
    3237.60A, SRMNormalFRI, Disc DrusenNormalNPIANormalNPI
    3544.00A, PRISRMFRINormalNPIANormalNPI
    3623.92ANormalRR, FRNormalNPIRI, FRNormalNPI
    4833.70A, FE*NormalRINormalNPIFRNormalNPI

    W-OCT vs. M-OCT

    Macular alterations on M-OCT and W-OCT presented as photoreceptor and RPE atrophy (n = 34), foveal or perifoveal subretinal material (SRM; n = 8), a preserved foveal island with perifoveal atrophy (n = 4), a combination of atrophy and SRM (n = 3), a combination of atrophy and unilateral foveal focal choroidal excavation (n = 2), and bilateral foveal cavitation (n = 1) [Figures 1-7]. In one patient (#52), a combination of atrophy and SRM was seen on the right eye, whereas the left eye showed SRM but no atrophic lesions. In addition, unilateral midperipheral focal choroidal excavation was observed in another patient [Figure 6].

    Figure 1. Patient #33 (49.8 years) with mild ABCA4-IRD with limited alterations of the foveal area. (A) FAF: flecks with increased intensity at the border of the macula as well as superior to the optic disc. (B) NIA: mostly flecks with reduced intensity, including the fovea, and a few flecks with slightly increased intensity. (C) W-OCT: the horizontal green arrow on the fundus image indicates the location of the B-scan. The blue arrow indicates a fleck of outer segment loss, corresponding to a barely detectable loss of FAF intensity (A) and a marked loss of NIA intensity (B). (D) W-OCT: near the upper temporal vascular arcade flecks of subretinal material (SRM, green arrows) correspond to flecks of increased FAF intensity (A) and reduced NIA intensity (B).

    Figure 2. Patient #45 (33.2 years) with moderate ABCA4-IRD with perifoveal atrophy. (A) FAF: multiple flecks with increased intensity in the macula as well as beyond the vascular arcades with peripapillary sparing, in addition to a parafoveal area of slightly increased intensity. (B) NIA: multiple flecks with reduced intensity, including the fovea and a parafoveal ring with peripapillary sparing, as well as a few flecks with slightly increased intensity. (C) W-OCT: paracentral loss of outer retinal layers corresponds to slightly increased FAF intensity (A) and a marked loss of NIA intensity (B). (D) W-OCT: near the upper temporal vascular arcade, flecks of subretinal material (SRM, blue arrow) correspond to flecks of increased FAF intensity (A) and reduced NIA intensity (B).

    Figure 3. Patient #1 (29.5 years) with marked ABCA4-IRD with macular atrophy. (A) FAF: multiple flecks with mostly increased intensity in the macula as well as beyond the vascular arcades with peripapillary sparing, in addition to central flecks of reduced intensity. (B) NIA: multiple flecks with markedly reduced intensity with peripapillary sparing and no flecks with increased intensity. (C) W-OCT: central loss of outer retinal layers as well as outer retinal irregularities nasal to the optic disc corresponding to FAF lesions with increased or reduced intensity (A) as well as NIA lesions with markedly reduced intensity.

    Figure 4. Patient #46 (8.8 years) with severe ABCA4-IRD with macular and widespread peripheral atrophy. (A) FAF: centrally reduced intensity in an oval area as well as fleck-like variable intensity with only partial peripapillary sparing. (B) NIA: severely reduced intensity at the posterior pole and slightly less distinct beyond the vascular arcades. (C) W-OCT: severe irregularities of the outer retinal layers with few flecks of subretinal material.

    Figure 5. Patient #31 (43.8 years) with severely progressed ABCA4-IRD with widespread scalloped atrophy. (A) FAF: multiple areas of absent FAF intensity bordered by areas with fleck-like reduced or increased intensity without peripapillary sparing, in addition to a parafoveal area of slightly increased intensity. (B) NIA: no measurable intensity. (C) W-OCT: increased reflectance in all retinal layers in the fovea. Marked disintegration of retinal layers over the whole length of the scan.

    Figure 6. Patient #12 [22.6 (left) and 26.4 years (right)] with severe ABCA4-IRD with macular and peripheral atrophy. (A) FAF: multiple flecks with increased or reduced intensity with peripapillary sparing. (B) NIA: centrally large areas and towards the periphery flecks with reduced intensity, peripapillary sparing. (C) W-OCT: subretinal material (blue arrow) corresponds to areas of reduced FAF (A) and NIA (B) intensity which enlarge during follow-up. The choroidal excavation outside of the macula (green arrows) progressed as well as indicated by the absence of the line of increased reflectance towards the choroid in this and adjacent scans at the follow-up examination.

    Figure 7. Patient #27 (14.3 and 18.8 years) with progressive ABCA4-IRD. W-OCT, central horizontal scan: (A) Disintegration of the outer retinal layers in the foveal and parafoveal region. (B) Progressed disintegration in the foveal area as well as peripheral progression of disintegration beyond the macular area (4 years later). W-OCT, superior horizontal scan tangentially to the upper temporal vascular arcade: (C) Normal structure of retinal layers. (D) Flecks of irregular outer retinal layers and SRM (4 years later).

    In 15/52 patients (28.8%), on both eyes no lesions beyond the area covered by M-OCT were observed either at the initial examination or at follow-up visits. In 37/52 patients (71.2%), retinal alterations extended beyond the area covered by M-OCT, which was unilateral only in one patient (#38). The areas involved included temporal extension of macular involvement, lesions nasal of the disc, lesions beyond the vascular arcades, or lesions continuous towards the periphery. The severity of findings varied as SRM (n = 16), atrophic lesions and SRM (n = 15), focal atrophic lesions (n = 3), marked outer retinal disorganization (n = 2), and marked outer retinal disorganization in a scalloped pattern (n = 1). In addition, W-OCT revealed bilateral dome-shaped bulbus configuration, marked posterior staphyloma, or optic disc drusen in one patient each.

    W-OCT vs. W-FAF

    In this series of 52 patients, W-FAF was obtained in all patients, whereas W-NIA and M-NIA could only be obtained in 49/52 patients. W-OCT did not identify lesions beyond the area covered by M-OCT that were not detected by W-FAF or W-NIA as well except for one patient (#35) with SRM in areas of normal W-FAF and W-NIA. The retinal characteristics of the lesions identified on FAF or NIA, e.g., whether there was focal atrophy or SRM, could be documented and followed over time by W-OCT [Figures 1-6]. Especially midperipheral focal choroidal excavation could not be identified on either W-FAF or W-NIA [Figure 6].

    M-FAF lesions consisted of flecks of both increased and reduced intensity (20/52), flecks of increased intensity (13/52), flecks of reduced intensity (4/52), large atrophic areas (3/52), atrophic areas and flecks of both increased and reduced intensity (2/52), atrophic areas and flecks of increased intensity (1/52), a parafoveal ring with increased intensity (4/52), and a ring and flecks of either increased (3/52) or reduced intensity (2/52). W-FAF was normal in 15 patients. Peripheral lesions consisted of flecks of increased intensity (19/52), flecks of both increased and reduced intensity (9/52), flecks of reduced intensity (3/52), atrophic areas and flecks of both increased and reduced intensity (1/52), atrophic areas and flecks of increased intensity (3/52), and rings with increased intensity (2/52). FAF lesions with increased intensity corresponded in general to W-OCT lesions with SRM, whereas FAF lesions with reduced intensity corresponded to W-OCT lesions with either intact or atrophic outer retinal layers. Preservation of the peripapillary region (peripapillary sparing) could not be defined in patients with lesions confined to the macular region (15/52) and was noted in the majority of patients with peripheral lesions (29/37). However, 8/37 patients did not show peripapillary sparing with lesions in contact to the optic disc.

    W-OCT vs. W-NIA

    NIA identified more extensive lesions compared to FAF [Figures 1-6]. M-NIA lesions consisted of flecks of both increased and reduced intensity (16/49), generally reduced intensity (10/49), flecks of reduced intensity (8/49), large atrophic areas (7/49), a parafoveal ring with increased intensity (3/49), a parafoveal ring with increased intensity and flecks of reduced intensity (2/49), flecks of increased intensity (1/49), atrophic areas and flecks of both increased and reduced intensity (1/49), or atrophic areas and flecks of reduced intensity (1/49). W-NIA was normal in 10/49 patients. Lesions beyond the vascular arcades consisted of flecks of reduced intensity (20/49), generally reduced intensity (14/49), and flecks of both increased and reduced intensity (5/49). NIA lesions with reduced intensity corresponded to either W-OCT lesions with SRM or atrophic outer retinal layers, indicating that different information is obtained by NIA compared to FAF. Therefore, a combination of FAF, NIA, and OCT provides the most detailed information on retinal lesions. Whereas the majority of patients showed peripapillary sparing on NIA as well (29/47), three patients with peripapillary sparing on FAF showed lesions adjacent to the optic disc on NIA [Figure 1].

    W-OCT follow up

    One or more W-OCT follow-up examinations were performed in 16/52 patients (30.8%) about 1-4 years after the initial examination. The majority (14/16) developed peripheral progression with involvement of more peripheral areas [Figure 7]. One patient with only macular lesions showed a slight increase of lesions size over a period of three years. The patient with bilateral foveal cavitation presented no alterations of the lesion after a period of three years.

    W-OCT and ABCA4 variants

    Out of the 52 patients, 48 carried either ≥ 2 (likely) pathogenic ABCA4 variants or the frequent hypomorphic c.5603A>T allele in combination with a (likely) pathogenic ABCA4 variant. In 4 patients, a single heterozygous (likely) pathogenic ABCA4 sequence change was identified (partially solved) (Group 1, Table 3). Peripheral lesions detected by W-OCT were less frequently observed in the 20 patients carrying the frequent mild alleles c.5603A>T or c.5882G>A in combination with a (likely) pathogenic ABCA4 variant (13 without vs. 7 with W-OCT lesions, Group 2, Table 3). This difference was statistically significant (Fisher’s exact test, P-value = 4.02 × 10-7). In contrast, all 28 patients carrying ≥ 2 (likely) pathogenic ABCA4 variants (Group 3, Table 3) displayed W-OCT lesions. In Group 3, the mean age of patients was 34.10 ± 15.47 years. In Group 2, the mean age of patients without W-OCT lesions was significantly lower (t test, P-value = 0.0035) than the mean age of patients with W-OCT lesions (32.15 ± 5.76 years vs. 48.37 ± 9.79 years), indicating that patients carrying mild ABCA4 alleles may develop peripheral abnormalities later in life.

    Table 3

    Correlation of ABCA4 genotype with lesions detected only by W-OCT

    GroupAllel 1/Allel 2No. of patients without lesions (mean age ± SD)No. of patients with lesions (mean age ± SD)
    1Pathogenic/+2 (27.95 ± 26.09 year)2 (48.6 ± 28.43 year)
    2Pathogenic/mild13 (32.15 ± 5.76 year)7 (48.37 ± 9.79 year)
    3Pathogenic/pathogenic-28 (34.10 ± 15.47 year)


    Although there is agreement that areas covered by retinal imaging should be extended to the retinal periphery, a succinct definition of the term “wide-field” or “ultra-wide-field” does not exist. In the present study, W-OCT was used in comparison to the frequently used macular volume scan (M-OCT). The wide-field scan used in this study extended horizontally to 55 degrees (16.1 mm) and vertically to 25 degrees (7.3 mm) as provided by the Heidelberg Engineering Spectralis design. Other manufacturers provide swept-source OCTs with a 12 mm × 9 mm field[20,30]. Future developments for OCTs intend to optimize resolution as well as extend the field of view[38,39].

    Examining larger retinal areas requires longer examination times, and therefore sufficient fixation and increased independence of eye movement are important. With the present technique, in 50/52 patients, high-resolution W-OCTs could be obtained. This is important, as the majority of patients had reduced visual acuity and problems with fixation. The present study showed that W-OCT is a feasible method for the examination of ABCA4-IRD patients.

    W-OCT identified retinal lesions outside the area covered by M-OCT in the majority of patients. Patients without W-OCT abnormalities carried either a single causative ABCA4 mutation or a mild ABCA4 variant in combination with a severe variant, which is consistent with previous observations that the presence of mild alleles results in a late-onset and/or milder phenotype[40].

    Although, except for one patient, the more peripheral W-OCT lesions were also identified by W-FAF and W-NIA, W-OCT added important information, not in respect to the location of the lesion but regarding the intraretinal alterations corresponding to W-FAF and W-NIA findings. Therefore, W-OCT provides an additional tool for disease definition and documentation of progression during follow up. Especially for treatment trials, it will be important to evaluate areas not covered by M-OCT to define markers for monitoring of treatment effects.

    In addition, as shown previously in myopic eyes[30], W-OCT allows identifying posterior staphyloma or dome-shaped bulbus configuration in myopic eyes. Within this series, foveal cavitation and optic disc drusen were seen in only one patient each (1.9%). In contrast to BEST1-associated retinal dystrophies[41], focal choroidal excavation was observed at the posterior pole in only two eyes of two ABCA4-IRD patients and in addition in the mid-periphery in a another eye of one patient (3/104; 2.8%), supporting that it is rare in ABCA4-associated disorders[42].

    The findings resulting from FAF and NIA evaluation are comparable to previous reports, indicating that NIA findings usually are more progressed and severe compared to FAF findings[5-8]. The frequency of peripapillary sparing was within the range reported previously[43], indicating that peripapillary sparing may lead to suspect ABCA4-IRD but is not a characteristic biomarker.


    Authors’ contributions

    Made substantial contributions to conception and design of the study and performed data analysis and interpretation: Saleh M, Stöhr H, Kiel C, Weber BHF, Kellner U

    Performed data acquisition, as well as provided administrative, technical, and material support: Kellner S, Weinitz S, Farmand G, Lommatsch A, Weber BHF, Kellner U

    Availability of data and materials

    Data cannot be shared as they contain patient specific information.

    Financial support and sponsorship


    Conflicts of interest

    All authors declared that there are no conflicts of interest.

    Ethical approval and consent to participate

    The ethics committee of the Ärztekammer Nordrhein, Düsseldorf, Germany approved the research.

    Consent for publication

    Not applicable.


    © The Author(s) 2021.


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    Cite This Article

    Saleh M, Stöhr H, Kiel C, Kellner S, Weinitz S, Farmand G, Weber BHF, Lommatzsch A, Kellner U. Wide-field optical coherence tomography in ABCA4-associated inherited retinal dystrophies. J Transl Genet Genom 2021;5:250-264.




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