fig2

Figure 2. A and B: 9.5 GHz, 12 K, ∂²χ”/∂B₀² EPR spectra from flash-frozen tissue samples of w/t rats over the entire spectral region (A) and around g_eff = 2 (B). Black traces are experimental data and red traces are computer simulations from Levenberg-Marquardt fitting; C: histogram obtained from Levenberg-Marquardt fitting of the liver spectrum to 18 distinct MRC redox centers; D: correlation plot highlighting pairs of redox centers with highly correlated calculated contributions to the overall EPR spectrum. Areas of very high correlation (dark blue) indicate that the fitting can be satisfied by an arbitrary linear combination of the two correlated signals, e.g., for two signals A and B, the fit is satisfied by xA +yB where the value of (x + y) is important but the individual values of x and y are not