fig5

Use of “default” parameter settings when analyzing single cell RNA sequencing data using Seurat: a biologist's perspective

Figure 5. UMAP plots of clustering solutions using scale factors of: 10,000 (baseline) (A, D); 1000 (B, E); and 100,000 (C, F). The cells are colored by: cluster (A-C); and cell type (D-F). Red circles and arrows indicate cluster gained when scale factor was reduced to 1000 (B, E) and cluster lost when increasing scale factor to 100,000 (C, E). Boxes below (D-F) show enlarged versions of the clusters within the red circles. UMAP: uniform manifold approximation and projection

Journal of Translational Genetics and Genomics
ISSN 2578-5281 (Online)
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