fig4

Figure 4. Kavalactones inhibits LSD1 enzyme activity. (A, B) Autodocktools program was used to dock kawain and methysticin with LSD1 protein, and Pymol program was used to observe the superimposed binding of kawain and methysticin to LSD1, respectively. 2D molecular structures of kawain and methysticin are shown to interact with key amino acids within the predicted binding site. (C) In vitro LSD1 enzyme activities were analyzed using LSD1 inhibitor screening kit. (D) Western blotting analysis of LSD1 expression and H3K9 dimethylation. LSD1: Lysine-specific demethylase 1.