fig1

Figure 1. Characterization of a new X. laevis adRP model. (A) Rho.L WT and Rho.LΔ11Δ1 sequences, beginning with the translation start site. The deleted (11 + 1) bases are indicated with dashes. The Sg6 target site is underlined in green. The Sg5 target site is underlined in blue. Corresponding predicted cut sites are indicated by scissors icons. (B) Dot blot assay for total rod opsin in a population derived from a mating between WT and WT/Rho.LΔ11Δ1 genotypes shows that two distinct groups are present with high and low rod opsin levels. Detection was with mAbB630N. Each data point represents a different animal. Five animals with high rod opsin levels and five with low rod opsin levels were genotyped (colored dots). Green were identified as WT, red were identified as WT/Rho.LΔ11Δ1. Black: not genotyped. (C) Confocal micrographs comparing retinal structure between WT and WT/Rho.LΔ11Δ1 genotypes. RD was observed in WT/Rho.LΔ11Δ1 animals relative to WT. Green: mabB630N (rod opsin); red: WGA; blue: Hoechst dye.