fig2

Figure 2. Single guide and double-guide treatment outcomes assayed by dot blot and confocal microscopy. (A-E) Total rod opsin levels assayed by dot blot using mabB630N anti-rod opsin antibody. Rod P values were determined by Kruskal-Wallis followed by Dunn’s test for multiple comparisons compare groups. P values for Dunn’s test are shown on the plots, P value for the Kruskal-Wallis test is given below (A) WT/WT (n = 17), WT/WT-Sg5 (n = 10), WT/WT-Sg2+5 (n = 5) (P = 0.051). (B) WT/Rho.LΔ11Δ1 (n = 8), WT/Rho.LΔ11Δ1-Sg5 (n = 19), WT/Rho.LΔ11Δ1-Sg2+5 (n = 11) (P = 0.00011). (C) WT/WT (n = 13), WT/WT-Sg6 (n = 12), WT/WT-Sg2+6 (n = 17) (P = 0.0014). (D) WT/Rho.LΔ11Δ1 (n = 11), WT/Rho.LΔ11Δ1-Sg6 (n = 11), WT/Rho.LΔ11Δ1-Sg2+6 (n = 7) (P = 0.0011). (E) Significant RD observed in Rho.LΔ11Δ1 animals relative to WT was prevented in groups treated with single and double guides, while no RD was observed in treated WT animals. Confocal micrographs are from representative retinas from each group. Green: mabB630N (rod opsin); red: WGA; blue: Hoechst dye.