fig4

Figure 4. Electroretinography and histology of nine-month-old animals. (A, B) WT/Rho.LΔ11Δ1 (red, n = 10) comparison with WT/WT (black, n = 6). (A) Mean a-wave amplitude: two-way ANOVA indicates a flash intensity effect (P = 0.0027), and a genotype effect (P = 0.0059). (B) Mean b-wave amplitude: two-way ANOVA indicates a flash intensity effect (P < 0.0001), but no effect of genotype. (C, D) WT/Rho.LΔ11Δ1 (red, n = 8) comparison with WT/Rho.LΔ11Δ1-Sg5 (black, n = 5). (C) Mean a-wave amplitude: two-way ANOVA indicates a flash intensity effect (P < 0.0001), and a treatment effect (P = 0.0001). (D) Mean b-wave amplitude: two-way ANOVA indicates a flash intensity effect (P < 0.0001), but no effect of genotype. Data presented as mean amplitude ± SEM in response to different light intensities. P values were determined by two-way ANOVA. (E) Confocal micrographs of retinal sections from 9-month old animals. RD is apparent in untreated 9-month-old WT/Rho.LΔ11Δ1 animals. Sg5-treated WT/Rho.LΔ11Δ1 animals are indistinguishable from WT animals. Green: mabB630N (rod opsin); red: WGA; blue: Hoechst dye.