fig5

Figure 5. Comparison of HDR and NHEJ-based treatments. (A) Total rod opsin levels assayed by dot blot using mabB630N antibody. The genotypes of a subset of animals were determined by Sanger sequencing. Green: WT/WT, red: derived from WT/Rho.LΔ11Δ1, black: not sequenced. P values were determined by Kruskal-Wallis followed by Dunn’s multiple comparisons test (n = 32 per group). For the Kruskal Wallis test, P = 0.017; values for Dunn’s tests are shown on the plot. (B) Confocal micrographs of retinal sections from corresponding untreated, Sg5-treated, and Sg5+ssDNA treated animals. RD observed in WT/Rho.LΔ11Δ1 animals was prevented in both treatment groups. Red: WGA; blue: Hoechst dye. (C) Confocal micrograph showing a mab2B2-positive cell, indicating successful homologous recombination of the ssDNA template. The single cell indicated by the arrowheads is shown at several magnifications. The bottom panels show different optical sections focused on the inner and outer segments, respectively. Green: mab2B2; Red: WGA; blue: Hoechst dye.