fig2

Figure 2. Schematic demonstration of miRNA-mediated inhibition of mRNAs. The GW182 protein interacts with AGO2 protein assembling the miRISC complex, which may enable the deadenylation and mRNAs degradation by PABP and APRO proteins with the CAF1-CCR4-NOT1 complex. The CAF1-CCR4-NOT1 complex is recruited to the 3’ UTR of specific mRNAs through interaction with PABP protein. APRO proteins may also associate with the PABP protein and recruit the CAF1-CCR4-NOT1 complex. Consequently, miRNAs could play dynamic roles in regulating mRNA expression via the decapping, translational inhibition, deadenylation, and degradation of mRNAs. The hammerhead represents inhibition. Note that some critical pathways have been omitted for clarity. AGO2: Argonaute2; PABP: poly(A)-binding protein; APRO: anti-proliferative; ORF: open reading frame; ROS: reactive oxygen species; miRISC: microRNA-induced silencing complex; AUG: initiating codon ATG; CAF1: chromatin assembly factor-1; CCR4-NOT: carbon catabolite repression 4-negative on TATA-less.